GC Content Calculator

🧬

GC Content Calculator

Calculate GC content, GC/AT ratio, and nucleotide composition of DNA or RNA sequences. Essential for primer design and hybridization studies.

Nucleic Acid Type *

Options

Sequence *

Enter sequence using A, T, G, C (DNA) or A, U, G, C (RNA)

Show Steps

⚠️

GC Content Result

—

📊

Nucleotide Composition

Visual representation of nucleotide distribution

—

📋

Calculation Breakdown

Step-by-step GC content calculation

🧬

GC Content

—

📊

GC/AT Ratio

—

(G+C)/(A+T)

🔢

Sequence Length

—

🌡️

Melting Temp (Tm)

—

°C

🧪

A Count

—

Adenine

🧪

T/U Count

—

Thymine/Uracil

🧪

G Count

—

Guanine

🧪

C Count

—

Cytosine

📊

Purines (A+G)

—

📊

Pyrimidines (T+C)

—

📊

Purine/Pyrimidine

—

Ratio

🔬

GC Skew

—

(G-C)/(G+C)

📖

GC Content Formulas

Key molecular biology concepts

💡

GC Content Tips

Understanding GC content

🧬 Scientific Disclaimer: This GC content calculator uses standard nucleotide weights. Results are for educational purposes. Actual GC content may vary with sequence modifications.